BRAF Codon 600 Mutation Detection with Reflex to MLH1 Promoter Methylation
Ordering Recommendation

Recommended reflex test to differentiate between Lynch syndrome and sporadic colorectal cancer in tumors showing loss of MLH1.

Polymerase Chain Reaction/Pyrosequencing
DNA isolation: Sun-Sat
Assay: Mon, Wed, Fri
5-10 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
Specimen Required
Patient Preparation
Tumor tissue. 
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Tissue block will be returned after testing.
Transport tissue block or 5 unstained 5-micron slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. (Min: 3 slides). Protect paraffin block and/or slides from excessive heat. 
Storage/Transport Temperature
Room temperature or refrigerated. Ship in cooled container during summer months. 
Unacceptable Conditions
Less than 25 percent tumor. Specimens fixed/processed in alternative fixatives (alcohol, Prefer) or heavy metal fixatives. Decalcified specimens. 
Include surgical pathology report. 
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable 
Reference Interval
Test Number
Reference Interval
 BRAF codon 600 Mutation DetectionBy report
 MLH1 Promoter MethylationBy report

Interpretive Data
Refer to report.

This test is performed pursuant to an agreement with Roche Molecular Systems, Inc.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

If BRAF codon 600 Mutation Detection is negative, then MLH1 Promoter Methylation will be added. Additional charges apply.
Hotline History
CPT Code(s)
88381; 81210; If reflexed, add 81288
Component Test Code*Component Chart NameLOINC
0051751BRAF codon 600 Mutation Detection
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • BRAF Gene Mutation Assay
  • EGFR targeted therapy
  • MLH1
  • sporadic Lynch Syndrome
  • V600E