Microsatellite Instability (MSI), HNPCC/Lynch Syndrome, by PCR
0051740
Ordering Recommendation
First-line screening test for LS. Directs additional molecular diagnostic testing for LS.
Submit With Order
Mnemonic
MSI PCR
Methodology
Polymerase Chain Reaction/Fragment Analysis
Performed
DNA isolation: Sun-Sat
Assay:
Sun, Tue, Thu
Reported
10-14 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Tumor AND normal epithelial tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Protect from excessive heat. Transport tissue block(s) or 10 unstained 5-micron slides (5 tumor and 5 normal epithelial). (Min: 3 tumor tissue and 3 normal epithelial tissue slides) Transport block(s) and/or slide(s) in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.  
Storage/Transport Temperature
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months  
Unacceptable Conditions
Specimens fixed/processed in alternative fixatives (alcohol, Prefer). Frozen specimens. Decalcified specimens. Less than 25 percent tumor or less than 50 percent normal epithelial tissue.  
Remarks
Include surgical pathology report. Tissue block will be returned after testing.  
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report.

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
88381; 81301
Components
Component Test Code*Component Chart Name
0051741Microsatellite Marker BAT-25
0051742Microsatellite Marker BAT-26
0051743Microsatellite Marker MONO-27
0051744Microsatellite Interpretation
0051745Microsatellite Marker NR-21
0051746Microsatellite Marker NR-24
2001370Microsatellite Instability Specimen
2002148Block ID
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • BAT -25, BAT-26, MON-27, NR-21, NR-24 genotypes
  • HNPCC MSI
  • HNPCC tumor screening
  • Lynch Syndrome MSI
  • Lynch syndrome tumor screening
  • microsatellite instability
  • MSI
  • MSI Lynch syndrome