EWSR1-FLI1 and EWSR1-ERG Translocations by RT-PCR (Inactive as of 11/14/16. Refer to 2007225)
Ordering Recommendation

Confirm histological diagnosis of Ewing sarcoma by detection of EWSR1-FLI1 and EWSR1-ERG fusions.

Reverse Transcription Polymerase Chain Reaction
RNA Isolation: Sun-Sat; Assay: Varies
3-7 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Specimen Preparation
Freeze immediately. Transport 100 mg or 0.5-2.0 cm3 fresh tissue in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect or contact ARUP Client Services at (800) 522-2787.
Also acceptable: Formalin fix (10 percent neutral buffered formalin) and paraffin embed tissue. Transport tissue block or 4 tissue scrolls (tissue shavings), 10 microns thick in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect or contact ARUP Client Services at (800) 522-2787. Protect paraffin block from excessive heat. Tissue block will be returned after testing.  
Storage/Transport Temperature
Fresh Tissue: CRITICAL FROZEN. Separate specimens must be submitted when multiple tests are ordered.
Room temperature. Also acceptable: Refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Specimens fixed/processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Slides. Decalcified tissue. Paraffin section slides.  
Fresh Tissue: Ambient: Unacceptable; Refrigerated: Unacceptable; Frozen: 1 year
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
By report
Interpretive Data
Refer to report.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.
CPT Code(s)
81401 x2
Component Test Code*Component Chart NameLOINC
0051219EWSR1-FLI1 and EWSR1-ERG by RT-PCR
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • ERG
  • ewing sarcoma breakpoint
  • EWS
  • EWS-FL1 Type 1
  • EWS-FLI1 type 2
  • EWSC
  • EWSR1-FL1 type 1
  • EWSR1-FL1 type 2
  • FLI1
  • FLI1 type 1
  • FLI1 type 2
  • FLI2
  • t(21;22)