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MPO/PR-3 (ANCA) Antibodies
0050707
Ordering Recommendation

Use to monitor previously established MPO/PR-3 antibodies or confirm an IFA ANCA  positive test result. Test does not include ANCA testing by IFA.
For  ANCA  testing, refer to the available panel tests. For the workup of suspected vasculitis, refer to ANCA-Associated Vasculitis Profile (ANCA/MPO/PR-3) with Reflex to ANCA Titer (2006480). For patients with a history of vasculitis, refer to MPO/PR-3 (ANCA) Antibodies (2002068).

Mnemonic
MPO/PR3
Methodology
Semi-Quantitative Multiplex Bead Assay
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.2 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma, urine, or other body fluids.  Contaminated, hemolyzed, or severely lipemic specimens. 
Remarks
 
Stability
After separation from cells: Ambient: 48 hours; Refrigerated: 2 weeks; Frozen: 1 year (avoid repeated freeze/thaw cycles) 
Reference Interval
Test Number
Components
Reference Interval
0050526Myeloperoxidase AntibodyNegative: 19 AU/mL or less
Equivocal: 20-25 AU/mL
Positive: 26 AU/mL or greater
0050527Serine Protease 3 AntibodyNegative: 19 AU/mL or less
Equivocal: 20-25 AU/mL
Positive: 26 AU/mL or greater

Interpretive Data
Approximately 90% of patients with a P-ANCA pattern by IFA have antibodies specific for MPO.
Approximately 85% of patients with a C-ANCA pattern by IFA have antibodies specific for PR3.

Note
CPT Code(s)
83516 x2
Components
Component Test Code*Component Chart NameLOINC
0050526Myeloperox Antibodies, IgG6969-0
0050527Serine Protease3, IgG6968-2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • 3-ANCA
  • ANCA Antibodies
  • Anticytoplasmic Autoantibodies
  • c-ANCA
  • MPO
  • MPO-ANCA
  • Myeloperoxidase Antibodies
  • p-ANCA
  • PR3
  • PR3-ANCA
  • Proteinase 3 Antibodies
  • Serine Protease 3