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Protein Electrophoresis, Serum
0050640
Ordering Recommendation

Aid in the diagnosis and management of monoclonal gammopathies, including multiple myeloma. Use to detect and quantify serum monoclonal protein. 

 

Mnemonic
SPEP
Methodology
Quantitative Capillary Electrophoresis
Performed
Mon-Fri
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube. 
Specimen Preparation
Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL) 
Storage/Transport Temperature
Refrigerated. 
Unacceptable Conditions
Plasma. 
Remarks
 
Stability
After separation from cells: Ambient: 8 hours; Refrigerated: 1 week; Frozen: 1 month 
Reference Interval
Test Number
Components
Reference Interval
 Total Protein-Electrophoresis6.00-8.30 g/dL
 Albumin3.75-5.01 g/dL
 Alpha 1 Globulin0.19-0.46 g/dL
 Alpha 2 Globulin0.48-1.05 g/dL
 Beta Globulin0.48-1.10 g/dL
 Gamma0.62-1.51 g/dL

Interpretive Data
Serum protein electrophoresis, when used as a screening test, is useful in the detection of various pathophysiologic states such as inflammation, protein loss, gammopathies, and other dysproteinemias. However, immunofixation electrophoresis (IFE) is a more sensitive technique for the identification of small M-proteins found in patients with amyloidosis, early or treated myeloma or macroglobulinemia, solitary plasmacytoma, or extramedullary plasmacytoma.

Note
A copy of the graph will follow final report.
For CSF electrophoresis, refer to Protein Electrophoresis, CSF (ARUP test code 0050590).
Hotline History
N/A
Components
Component Test Code*Component Chart NameLOINC
0050020Albumin2862-1
0050035Alpha 1 Globulin2865-4
0050060Alpha 2 Globulin2868-8
0050110Beta Globulin2871-2
0050270Gamma2874-6
0050545Total Protein-Electrophoresis2885-2
2005168SPEP/IFE Interpretation49275-1
2012453EER Protein Electrophoresis, Serum
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Protein ELP
  • Serum Protein Electrophoresis
  • SPEP