Kappa and Lambda Free Light Chains (Bence Jones Protein), Quantitative, Urine
Ordering Recommendation
Aids in the diagnosis and management of multiple myeloma and related disorders. Preferred test to detect and quantify urinary free light chains (Bence Jones protein).
Qualitative Immunofixation Electrophoresis/Quantitative Nephelometry
1-5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
24-hour urine. Refrigerate during collection. Also acceptable: Random urine specimens and urine supernate.  
Specimen Preparation
Transfer two 4 mL aliquots from a well-mixed 24-hour collection to individual ARUP Standard Transport Tubes. (Min: 3 mL) Record total volume and collection time interval on transport tube and test request form.  
Storage/Transport Temperature
Unacceptable Conditions
Ambient: 2 hours; Refrigerated: 1 week; Frozen: Unacceptable  
Reference Interval
Test Number Components Reference Interval
Total Protein 10-​140 mg/d
Albumin, Urine Detected
Alpha-​1 Globulins, Urine None detected
Alpha-​2 Globulins, Urine None detected
Beta Globulins, Urine None detected
Gamma, Urine None detected
Free Urinary Kappa Light Chains 0.14 -​ 2.42 mg/dL
Free Urinary Kappa Excretion/Day By report
Free Urinary Lambda Light Chain 0.02 -​ 0.67 mg/dL
Free Urinary Lambda Excretion/Day By report
Free Urinary Kappa/Lambda Ratio 2.04 -​ 10.37 (ratio)
IFE Interpretation By report
Interpretive Data
Total urinary protein is determined nephelometrically by adding the albumin and kappa and/or lambda light chains. This value may not agree with the total protein as determined by chemical methods, which characteristically underestimate urinary light chains.
CPT Code(s)
84156; 86335; 83883 x2
Component Test Code*Component Chart Name
0050025Albumin, Urine
0050050Alpha-1, Urine
0050055Alpha-2, Urine
0050120Urine Beta Globulin
0050285Gamma, Urine
0050565Total Protein
0050683Free Urinary Kappa Light Chains
0050684Free Urinary Kappa Excretion/Day
0050685Free Urinary Lambda Light Chain
0050686Free Urinary Lambda Excretion/Day
0050687Free Urinary Kappa/Lambda Ratio
0050688IFE Interpretation
0097110Total Volume
0097111Hours Collected
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • Free Kappa & Lambda Light Chains, IFE
  • Monoclonal Protein Study, Random Urine
  • Protein Electrophoresis