Mismatch Repair by Immunohistochemistry
0049302
 
Ordering Recommendation
Effective primary test for Lynch syndrome. Use to determine mismatch repair deficiency and to direct additional molecular diagnostic testing for Lynch syndrome.
Mnemonic
MSI
Methodology
Qualitative Immunohistochemistry
Performed
Mon, Thu
Reported
Within 5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Tumor tissue.  
Specimen Preparation
Formalin fix (10 percent neutral buffered formalin is preferred) and paraffin embed specimen. If sending precut slides, do not oven bake. Transport tissue block or 15 unstained (3-5 micron thick sections), positively charged slides in a tissue transport kit (ARUP supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787.(Min: 12 slides). Protect paraffin block and/or slides from excessive heat.  
Storage/Transport Temperature
Room temperature or refrigerated. Ship in cooled container during summer months.  
Unacceptable Conditions
Frozen specimens. Specimens fixed or processed in alternative fixatives (alcohol, Prefer®) or heavy metal fixatives (B-4 or B-5). Depleted or no tumor in tissue. Specimens submitted with non-representative tissue type.  
Remarks
Only tissue that is clearly carcinoma (established by histological criteria) should be tested. Include surgical pathology report. Submit electronic request. If you do not have electronic ordering capability, use an ARUP requisition form complete with an ARUP client number. For additional technical details, please contact ARUP Client Services at (800) 522-2787.  
Stability
Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Unacceptable  
Reference Interval
Interpretive Data
Refer to report

See Compliance Statement B: www.aruplab.com/CS
Note
CPT Code(s)
88342 x4
Components
Component Test Code*Component Chart Name
0049199Client Case or Ref #
0049303Mismatch Repair by IHC, Result
0049305Mismatch Repair by IHC with MLH1
0049310Mismatch Repair by IHC with MSH2
0049320Mismatch Repair by IHC with MSH6
0049325Mismatch Repair by IHC with PMS2
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, contact interface support at interface.support@aruplab.com.
Cross References
  • MLH1 IHC
  • MMR IHC
  • MMR Immunostaining
  • MSH2 IHC
  • MSH6 IHC
  • MSI
  • MSI Antibody Immunostaining
  • MSI IHC
  • PMS2 IHC