Esterase Stain, Nonspecific
0049050
Ordering Recommendation
Submit With Order
  • Anatomic Pathology Test Request Form Recommended (ARUP form #32960)
Mnemonic
EST-NS
Methodology
Cytochemical Stain
Performed
Mon-Fri
Reported
Within 5 days
New York DOH Approval Status
This test is New York DOH approved.
Specimen Required
Patient Preparation
 
Collect
Lavender (EDTA) or green (sodium or lithium heparin). OR heparinized bone marrow aspirate.  
Specimen Preparation
Blood: Protect from light. Transport 5 mL whole blood AND 6 unfixed, air-dried, and unstained push smears made from the blood submitted. (Min: 1 mL AND 6 unfixed smears).
OR Bone Marrow: Protect from light.
Transport 1 mL heparinized aspirate AND 6 unfixed, air-dried, and unstained bone marrow aspirate smears. (Min: 0.5 mL AND 6 unfixed smears).  
Storage/Transport Temperature
Room temperature. Specimens should be received within 24 hours of collection; testing must be performed within 48 hours of collection.  
Unacceptable Conditions
Frozen specimens. Peripheral blood smears older than one year. Anticoagulated blood or bone marrow sent without well-prepared unfixed smears older than 48 hours.  
Remarks
 
Stability
Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable  
Reference Interval
Refer to report.
Interpretive Data
Nonspecific Esterase stain (using alpha-naphthyl butyrate) is very useful in identifying monocytes found in acute monocytic leukemia (AML) types M4 and M5.
Note
CPT Code(s)
88319
Components
Component Test Code*Component Chart Name
0049050Esterase Stain, Nonspecific
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Cross References
  • Non-Specific Esterase
  • NSE
  • NSE Butyrate