JAK2 Gene, V617F Mutation, Quantitative
Ordering Recommendation

Quantitates JAK2 V617F allele frequency in enriched granulocytes from peripheral whole blood. Aids in risk stratification and therapeutic monitoring of JAK2 V617F mutation positive myeloproliferative neoplasms.

Polymerase Chain Reaction
DNA isolation: Sun-Sat
Mon, Wed, Fri
5-9 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Lavender (EDTA). Also acceptable: DNA extracted by CLIA certified lab. 
Specimen Preparation
Whole Blood: Do not freeze. Transport 5 mL whole blood. (Min: 1 mL).
Extracted DNA: Transport 40uL DNA with at least 50 ng/uL concentration (Min: 40uL). Transport DNA in a tissue transport kit (ARUP Supply #47808) available online through eSupply using ARUP Connect™ or contact ARUP Client Services at (800) 522-2787. 
Storage/Transport Temperature
Unacceptable Conditions
Plasma, serum, FFPE tissue blocks/slides, or frozen tissue, bone marrow, DNA extracted by a non-CLIA lab. Specimens collected in anticoagulants other than EDTA or sodium heparin. Clotted or grossly hemolyzed specimens. 
Ambient: 24 hours; Refrigerated: 48 hours; Frozen: Unacceptable
Extracted DNA: Ambient: 1 month; Refrigerate: Indefinitely; Frozen: Indefinitely 
Reference Interval
Interpretive Data
Refer to report.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
View Hotline History
Component Test Code*Component Chart NameLOINC
0040167JAK2 Gene, V617F Mutation, Quantitative53761-3
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • BCR-ABL1-negative tesing
  • Classic BCR-ABL1-negative MPN testing
  • MPN JAK 2
  • mutant JAK 2 V617F allelic burden