Iron, Liver
Ordering Recommendation

Useful in confirming hepatic iron overload, particularly in individuals with hemochromatosis and no common HFE mutations. Initial approach to diagnosis for hemochromatosis should include Iron and Iron Binding Capacity (0020420) (NOTE: test includes serum transferrin saturation) and Ferritin (0070065).

Quantitative Inductively Coupled Plasma-Mass Spectrometry
Mon, Wed, Thu, Fri, Sat
2-6 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Liver tissue obtained with an 18 gauge needle. 
Specimen Preparation
Transport at least a 1 cm long specimen. Tissue can be fresh, paraffin-embedded, or dried. Also acceptable: Formalin-fixed. Specimens should be stored and transported in a metal-free container such as a royal blue (no additive). 
Storage/Transport Temperature
Unacceptable Conditions
Specimens less than 0.25 mg (dry weight). Specimens stored or shipped in saline. 
Age is required on test request form in order to calculate iron index. 
Paraffin block, preserved (formalin), or dried: Ambient: Indefinitely; Refrigerated: Indefinitely; Frozen: Indefinitely
Fresh tissue: Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely 
Reference Interval
Hepatic Iron Concentration by Weight (HIC)200-2,000 µg/g of tissue200-1,600 µg/g of tissue
Hepatic Iron Index (HII)Less than 1.0Less than 1.0

Interpretive Data
A Hepatic Iron Index (HII) is not calculated for patients less than 14 years. An HII less than 1.0 is consistent with normal iron accumulation. An HII 1.0 through 1.9 is consistent with mild iron accumulation such as in heterozygous hemochromatosis or alcoholic liver disease. An HII greater than 1.9 is consistent with iron overload such as in homozygous hemochromatosis, porphyria cutanea tarda, and cirrhotic liver disease. The HII will decrease with chelation, chronic blood loss, or phlebotomy.

Compliance Statement B: For laboratory developed tests not using a RUO kit, and for FDA approved, cleared or 510(k) exempt assays with alterations. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

Hotline History
Component Test Code*Component Chart NameLOINC
0020815Tissue Weight29638-4
0020816Hepatic Iron Concentration by Weight15357-7
0020817Hepatic Iron Index49061-5
2004961EER Iron, Liver11526-1
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • Fe
  • Hepatic Iron Concentration
  • Hepatic Iron Index
  • Iron Tissue
  • Iron, Tissue Liver
  • Metals, Tissue
  • Quantitative Iron
  • Total Iron, Liver Tissue