Human Immunodeficiency Virus Type 1 (HIV-1) Antibody, Confirmation by Western Blot, with Reflex to HIV-2 Antibody
Ordering Recommendation

Order only if patient is positive (repeatedly reactive) for combined HIV-1 and HIV-2 antibody screen.

Qualitative Western Blot/Qualitative Enzyme-Linked Immunosorbent Assay/Qualitative Immunoassay
Sun, Tue-Sat
1-3 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
Serum separator tube. Also acceptable: Lt. blue (sodium citrate), green (sodium or lithium heparin) or lavender (EDTA). 
Specimen Preparation
Separate serum or plasma from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.5 mL) Remove particulate material. 
Storage/Transport Temperature
Unacceptable Conditions
Hemolyzed, or lipemic specimens. 
Ambient: Unacceptable; Refrigerated: 1 week; Frozen: Indefinitely (avoid repeated freeze/thaw cycles) 
Reference Interval
Test Number
Reference Interval
0020284Human Immunodeficiency Virus Type 1 (HIV-1) Antibody Confirmation by Western BlotNegative
HIV-2 Antibody by ELISANegative
HIV-2 Antibody, SupplementalNegative

Interpretive Data
This assay should not be used for blood donor screening, associated re-entry protocols, or for screening Human Cell, Tissues and Cellular Tissue-Based Products (HCT/P).

Order this test only when a specimen is repeatedly reactive for HIV-1 or HIV 1,2 antibodies.

If HIV-1 Antibody, Confirmation by Western Blot is Indeterminate, Negative or has Non-specific Staining, then HIV-2 Antibody by ELISA with Reflex to HIV-2 Supplemental will be added. Additional charges apply.

Refer to Human Immunodeficiency Virus 1 Antibody, Confirmation (0020284) and Human Immunodeficiency Virus Type 2 (HIV-2) Antibody by ELISA with Reflex to HIV-2 Supplemental (0051250) for additional information regarding Performed or Reported times.
CPT Code(s)
86689; if reflexed, add 86702; if reflexed, add 86702
Component Test Code*Component Chart NameLOINC
0020284HIV-1 Antibody Confirm, Western Blot5221-7
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
  • HIV 1 western blot, reflex to HIV 2 ELISA
  • HIV-1 Antibody Confirm, Western Blot
  • HIV1 reflex to HIV2