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Lactate Dehydrogenase, Isoenzymes
0020413
Ordering Recommendation

Do not use to detect myocardial injury. Troponin I (0090613) and Troponin T (0098803) are the recommended tests for diagnosis and management of acute coronary syndrome. In rare cases, this test may be used to evaluate elevated lactate dehydrogenase associated with non-cardiac muscle injury.

Mnemonic
LDISO
Methodology
Quantitative Enzymatic/Electrophoresis
Performed
Sun-Sat
Reported
1-2 days
New York DOH Approval Status
This test is New York DOH approved.
Submit With Order
ARUP Consult®
Disease Topics
Specimen Required
Patient Preparation
 
Collect
Serum separator tube (SST) or plain red. 
Specimen Preparation
Do not refrigerate or freeze. Allow specimen to clot completely at room temperature. Separate serum from cells ASAP or within 2 hours of collection. Transfer 1 mL serum to an ARUP Standard Transport Tube. (Min: 0.6 mL) 
Storage/Transport Temperature
Room temperature. 
Unacceptable Conditions
Specimens collected with EDTA, potassium oxalate, or sodium fluoride anticoagulants. Frozen, refrigerated, or hemolyzed specimens. 
Remarks
 
Stability
After separation of cells: Ambient: 1 week; Refrigerated: Unacceptable; Frozen: Unacceptable 
Reference Interval
LD-1: 14-27%
LD-2: 29-42%
LD-3: 18-30%
LD-4: 8-15%
LD-5: 6-23%

Lactate Dehydrogenase, Total:
0-1 month: 200-465 U/L
2-17 months: 200-450 U/L
18 months-10 years: 165-430 U/L
11-16 years: 127-287 U/L
17 years and older: 105-230 U/L
Interpretive Data


Note
LD-1 and LD-2 are elevated in hemolyzed specimens and serum which has not been separated from cells.  LD-3, LD-4, and LD-5 are labile at low temperatures, and are erroneously low in specimens that have been refrigerated or frozen.
CPT Code(s)
83625; 83615
Components
Component Test Code*Component Chart NameLOINC
0020015Lactate Dehydrogenase - 12536-1
0020016Lactate Dehydrogenase - 22539-5
0020017Lactate Dehydrogenase - 32542-9
0020018Lactate Dehydrogenase - 42545-2
0020019Lactate Dehydrogenase - 52548-6
0020107Lactate Dehydrogenase, Total14804-9
* Component test codes cannot be used to order tests. The information provided here is not sufficient for interface builds; for a complete test mix, please click the sidebar link to access the Interface Map.
Aliases
  • Isoenzymes
  • Isoenzymes of Lactate Dehydrogenase
  • Isoenzymes, LD
  • Lactate Dehydrogenase Isoenzymes, S
  • LD
  • LDH
  • LDH Fractionation
  • LDH Isoenzymes